Practical guide to ChIP-seq data analysis / Borbala Mifsud, Kathi Zarnack, Anais F Bardet.

By:

Mifsud, Borbala [author.]

Contributor(s):

Material type: Text

TextSeries: Focus computational biology seriesPublisher: Boca Raton, Florida : CRC Press, [2019]Description: 1 online resourceContent type:

text

Media type:

computer

Carrier type:

online resource

ISBN:

9780429487590
9780429946394
9781032241760

Subject(s):

Additional physical formats: Print version:: Practical guide to ChIP-seq data analysisDDC classification:

572.87 MIF 2019 23

LOC classification:

QH599

NLM classification:

QU 550

Summary: Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is amongst the most widely used methods in molecular biology. In order to confidently identify enriched regions in a ChIP-seq sample, the read distribution needs to be compared to a background distribution to control for potential biases. The optimal control for a ChIP-seq experiment is to sequence the input DNA purified from the sheared chromatin before the antibody incubation step. ChIP-seq is an experiment based on antibody immunoprecipitation performed on a population of cells to define protein binding sites in the genome using high-throughput sequencing (HTS). Since its first introduction in 2005, HTS has been a rapidly evolving field, resulting in a broad spectrum of available library preparation protocols and sequencing technologies. DNA adenine methyltransferase identification works as an alternative to chromatin immunoprecipitation. Since ChIP-seq is an antibody-based immunoprecipitation experiment, its efficiency strongly depends on the quality and specificity of the antibody.

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Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Book	Symbiosis International University, Dubai	572.87 MIF 2019 (Browse shelf(Opens below))	1	Available		SIU00299
Book	Symbiosis International University, Dubai	572.87 MIF 2019 (Browse shelf(Opens below))	2	Available		SIU00300

Includes bibliographical references and index.

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is amongst the most widely used methods in molecular biology. In order to confidently identify enriched regions in a ChIP-seq sample, the read distribution needs to be compared to a background distribution to control for potential biases. The optimal control for a ChIP-seq experiment is to sequence the input DNA purified from the sheared chromatin before the antibody incubation step. ChIP-seq is an experiment based on antibody immunoprecipitation performed on a population of cells to define protein binding sites in the genome using high-throughput sequencing (HTS). Since its first introduction in 2005, HTS has been a rapidly evolving field, resulting in a broad spectrum of available library preparation protocols and sequencing technologies. DNA adenine methyltransferase identification works as an alternative to chromatin immunoprecipitation. Since ChIP-seq is an antibody-based immunoprecipitation experiment, its efficiency strongly depends on the quality and specificity of the antibody.

Description based on print version record.

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