TY  - BOOK
AU  - Mifsud,Borbala
AU  - Zarnack,Kathi
AU  - Bardet,Anais
TI  - Practical guide to ChIP-seq data analysis
T2  - Focus computational biology series
AV  - QH599 
U1  - 572.87 MIF 2019 23
PY  - 2019///]
CY  - Boca Raton, Florida
PB  - CRC Press
KW  - Chromatin Immunoprecipitation
KW  - methods
KW  - High-Throughput Nucleotide Sequencing
KW  - Proteins
KW  - chemistry
N1  - Includes bibliographical references and index
N2  - Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is amongst the most widely used methods in molecular biology. In order to confidently identify enriched regions in a ChIP-seq sample, the read distribution needs to be compared to a background distribution to control for potential biases. The optimal control for a ChIP-seq experiment is to sequence the input DNA purified from the sheared chromatin before the antibody incubation step. ChIP-seq is an experiment based on antibody immunoprecipitation performed on a population of cells to define protein binding sites in the genome using high-throughput sequencing (HTS). Since its first introduction in 2005, HTS has been a rapidly evolving field, resulting in a broad spectrum of available library preparation protocols and sequencing technologies. DNA adenine methyltransferase identification works as an alternative to chromatin immunoprecipitation. Since ChIP-seq is an antibody-based immunoprecipitation experiment, its efficiency strongly depends on the quality and specificity of the antibody
ER  -