Practical guide to ChIP-seq data analysis / Borbala Mifsud, Kathi Zarnack, Anais F Bardet.

By:

Mifsud, Borbala [author.]

Contributor(s):

Material type: Text

TextSeries: Focus computational biology seriesPublisher: Boca Raton, Florida : CRC Press, [2019]Description: 1 online resourceContent type:

text

Media type:

computer

Carrier type:

online resource

ISBN:

9780429487590
9780429946394
9781032241760

Subject(s):

Additional physical formats: Print version:: Practical guide to ChIP-seq data analysisDDC classification:

572.87 MIF 2019 23

LOC classification:

QH599

NLM classification:

QU 550

Summary: Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is amongst the most widely used methods in molecular biology. In order to confidently identify enriched regions in a ChIP-seq sample, the read distribution needs to be compared to a background distribution to control for potential biases. The optimal control for a ChIP-seq experiment is to sequence the input DNA purified from the sheared chromatin before the antibody incubation step. ChIP-seq is an experiment based on antibody immunoprecipitation performed on a population of cells to define protein binding sites in the genome using high-throughput sequencing (HTS). Since its first introduction in 2005, HTS has been a rapidly evolving field, resulting in a broad spectrum of available library preparation protocols and sequencing technologies. DNA adenine methyltransferase identification works as an alternative to chromatin immunoprecipitation. Since ChIP-seq is an antibody-based immunoprecipitation experiment, its efficiency strongly depends on the quality and specificity of the antibody.

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Call number	Copy number	Status	Date due	Barcode
Book	Symbiosis International University, Dubai	572.87 MIF 2019 (Browse shelf(Opens below))	1	Available		SIU00299
Book	Symbiosis International University, Dubai	572.87 MIF 2019 (Browse shelf(Opens below))	2	Available		SIU00300

Browsing Symbiosis International University, Dubai shelves Close shelf browser (Hides shelf browser)

Previous								Next
Previous	519.536 BRE 1998 Classification and regression trees /	571.6028 LUC 2019 Essential computer graphics techniques for modeling, animating, and rendering biomolecules and cells : a guide for the scientist and artist /	571.6028 LUC 2019 Essential computer graphics techniques for modeling, animating, and rendering biomolecules and cells : a guide for the scientist and artist /	572.87 MIF 2019 Practical guide to ChIP-seq data analysis /	572.87 MIF 2019 Practical guide to ChIP-seq data analysis /	574.5 MOR 2006 An introduction to comparative psychology /	574.5 MOR 2006 An introduction to comparative psychology /	Next

Includes bibliographical references and index.

Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) is amongst the most widely used methods in molecular biology. In order to confidently identify enriched regions in a ChIP-seq sample, the read distribution needs to be compared to a background distribution to control for potential biases. The optimal control for a ChIP-seq experiment is to sequence the input DNA purified from the sheared chromatin before the antibody incubation step. ChIP-seq is an experiment based on antibody immunoprecipitation performed on a population of cells to define protein binding sites in the genome using high-throughput sequencing (HTS). Since its first introduction in 2005, HTS has been a rapidly evolving field, resulting in a broad spectrum of available library preparation protocols and sequencing technologies. DNA adenine methyltransferase identification works as an alternative to chromatin immunoprecipitation. Since ChIP-seq is an antibody-based immunoprecipitation experiment, its efficiency strongly depends on the quality and specificity of the antibody.

Description based on print version record.

There are no comments on this title.

to post a comment.